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Journal of Biomedical Optics • Open Access

Ex vivo imaging of human thyroid pathology using integrated optical coherence tomography and optical coherence microscopy
Author(s): Chao Zhou; Yihong Wang; Aaron D. Aguirre; Tsung-Han Tsai; David W. Cohen; James L. Connolly; James G. Fujimoto

Paper Abstract

We evaluate the feasibility of optical coherence tomography (OCT) and optical coherence microscopy (OCM) for imaging of benign and malignant thyroid lesions ex vivo using intrinsic optical contrast. 34 thyroid gland specimens are imaged from 17 patients, covering a spectrum of pathology ranging from normal thyroid to benign disease/neoplasms (multinodular colloid goiter, Hashimoto's thyroiditis, and follicular adenoma) and malignant thyroid tumors (papillary carcinoma and medullary carcinoma). Imaging is performed using an integrated OCT and OCM system, with <4 μm axial resolution (OCT and OCM), and 14 μm (OCT) and <2 μm (OCM) transverse resolution. The system allows seamless switching between low and high magnifications in a way similar to traditional microscopy. Good correspondence is observed between optical images and histological sections. Characteristic features that suggest malignant lesions, such as complex papillary architecture, microfollicules, psammomatous calcifications, or replacement of normal follicular architecture with sheets/nests of tumor cells, can be identified from OCT and OCM images and are clearly differentiable from normal or benign thyroid tissues. With further development of needle-based imaging probes, OCT and OCM could be promising techniques to use for the screening of thyroid nodules and to improve the diagnostic specificity of fine needle aspiration evaluation.

Paper Details

Date Published: 1 January 2010
PDF: 9 pages
J. Biomed. Opt. 15(1) 016001 doi: 10.1117/1.3306696
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Chao Zhou, Massachusetts Institute of Technology (United States)
Yihong Wang, Beth Israel Deaconess Medical Ctr. (United States)
Aaron D. Aguirre, Massachusetts Institute of Technology (United States)
Tsung-Han Tsai, Massachusetts Institute of Technology (United States)
David W. Cohen, Beth Israel Deaconess Medical Ctr. (United States)
James L. Connolly, Beth Israel Deaconess Medical Ctr. (United States)
James G. Fujimoto, Massachusetts Institute of Technology (United States)


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