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Journal of Biomedical Optics • Open Access

Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window
Author(s): Sven Meissner; Lilla Knels; Christian Schnabel; Thea Koch; Edmund Koch

Paper Abstract

In vivo determination of 3-D and dynamic geometries of alveolar structures with adequate resolution is essential for developing numerical models of the lung. A thorax window is prepared in anesthetized rabbits by removal of muscle tissue between the third and fourth rib without harming the parietal pleura. The transparent parietal pleura allows contact-free imaging by intravital microscopy (IVM) and 3-D optical coherence tomography (3-D OCT). We demonstrate that dislocation of the lung surface is small enough to observe identical regions in the expiratory and inspiratory plateau phase, and that OCT in this animal model is suitable for generating 3-D geometry of in vivo lung parenchyma. To our knowledge, we present a novel thorax window preparation technique for 3-D imaging of alveolar dynamics for the first time. The 3-D datasets of the fine structure of the lung beneath the pleura could provide a basis for the development of 3-D numerical models of the lung.

Paper Details

Date Published: 1 January 2010
PDF: 4 pages
J. Biomed. Opt. 15(1) 016030 doi: 10.1117/1.3302809
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Sven Meissner, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Lilla Knels, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Christian Schnabel, Technische Univ. Dresden (Germany)
Thea Koch, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Edmund Koch, Technische Univ. Dresden (Germany)

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