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Journal of Biomedical Optics • Open Access

Comparison of cobinamide to hydroxocobalamin in reversing cyanide physiologic effects in rabbits using diffuse optical spectroscopy monitoring
Author(s): Matthew Brenner; Sari B. Mahon; Jangwoen Lee; Jae Gwan Kim; David Sho Mukai; Seth Goodman; Kelly Ann Kreuter; Rebecca Ahdout; Othman Mohammad; Vijay S. Sharma; William Blackledge; Gerry R. Boss

Paper Abstract

Our purpose is to compare cobinamide to hydroxocobalamin in reversing cyanide (CN)-induced physiologic effects in an animal model using diffuse optical spectroscopy (DOS). Cyanide poisoning is a major threat worldwide. Cobinamide is a novel molecule that can bind two molecules of cyanide, has a much higher binding affinity than hydroxocobalamin, and is more water soluble. We investigated the ability of equimolar doses of cobinamide and hydroxocobalamin to reverse the effects of cyanide exposure in an animal model monitored continuously by DOS. Cyanide toxicity was induced in 16 New Zealand white rabbits by intravenous infusion. Animals were divided into three groups: controls (n=5) received saline following cyanide, hydroxocobalamin (N=6) following cyanide, and cobinamide (N=5) following cyanide. Cobinamide caused significantly faster and more complete recovery of oxy- and deoxyhemoglobin concentrations in cyanide-exposed animals than hydroxocobalamin- or saline-treated animals, with a recovery time constant of 13.8±7.1 min compared to 75.4±25.1 and 76.4±42.7 min, for hydroxocobalamin- and saline-treated animals, respectively (p<0.0001). This study indicates that cobinamide more rapidly and completely reverses the physiologic effects of cyanide than equimolar doses of cobalamin at the dose used in this study, and CN effects and response can be followed noninvasively using DOS.

Paper Details

Date Published: 1 January 2010
PDF: 8 pages
J. Biomed. Opt. 15(1) 017001 doi: 10.1117/1.3290816
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Matthew Brenner, Univ. of California, Irvine (United States)
Sari B. Mahon, Univ. of California, Irvine (United States)
Jangwoen Lee, Beckman Laser Institute and Medical Ctr. (United States)
Jae Gwan Kim, Beckman Laser Institute and Medical Ctr. (United States)
David Sho Mukai, Beckman Laser Institute and Medical Ctr. (United States)
Seth Goodman, Beckman Laser Institute and Medical Ctr. (United States)
Kelly Ann Kreuter, Beckman Laser Institute and Medical Ctr. (United States)
Rebecca Ahdout, Beckman Laser Institute and Medical Ctr. (United States)
Othman Mohammad, Univ. of California, San Diego (United States)
Vijay S. Sharma, Univ. of California, San Diego (United States)
William Blackledge, Univ. of California, San Diego (United States)
Gerry R. Boss, Univ. of California, San Diego (United States)


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