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Journal of Biomedical Optics • Open Access

Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry
Author(s): Natan Tzvi Shaked; John D. Finan; Farshid Guilak; Adam P. Wax

Paper Abstract

We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Reconstructed images are obtained by an efficient digital process. We use the resulting images to calculate quantitative temporal-spatial morphological parameters of the cell, with the observed dynamics limited only by the true frame rate of the camera. To show the utility of WFDI in recording articular chondrocyte dynamics, we also provide an experimental comparison of WFDI and differential interference contrast microscopy.

Paper Details

Date Published: 1 January 2010
PDF: 3 pages
J. Biomed. Opt. 15(1) 010505 doi: 10.1117/1.3290242
Published in: Journal of Biomedical Optics Volume 15, Issue 1
Show Author Affiliations
Natan Tzvi Shaked, Duke Univ. (United States)
John D. Finan, Duke Univ. (United States)
Farshid Guilak, Duke Univ. (United States)
Adam P. Wax, Duke Univ. (United States)

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