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Journal of Biomedical Optics

Is there a difference between T- and B-lymphocyte morphology?
Author(s): Dmitry I. Strokotov; Maxim A. Yurkin; Konstantin V. Gilev; Dirk R. Van Bockstaele; Alfons G. Hoekstra; Nikolay Rubtsov; Valerii Pavlovich Maltsev
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Paper Abstract

We characterize T- and B-lymphocytes from several donors, determining cell diameter, ratio of nucleus to cell diameter, and refractive index of the nucleus and cytoplasm for each individual cell. We measure light-scattering profiles with a scanning flow cytometer and invert the signals using a coated sphere as an optical model of the cell and by relying on a global optimization technique. The main difference in morphology of T- and B-lymphocytes is found to be the larger mean diameters of the latter. However, the difference is smaller than the natural biological variability of a single cell. We propose nuclear inhomogeneity as a possible reason for the deviation of measured light-scattering profiles from real lymphocytes from those obtained from the coated sphere model.

Paper Details

Date Published: 1 November 2009
PDF: 12 pages
J. Biomed. Opt. 14(6) 064036 doi: 10.1117/1.3275471
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Dmitry I. Strokotov, Institute of Chemical Kinetics and Combustion (Russian Federation)
Maxim A. Yurkin, Institute of Chemical Kinetics and Combustion (Russian Federation)
Konstantin V. Gilev, Institute of Chemical Kinetics and Combustion (Russian Federation)
Dirk R. Van Bockstaele, Esoterix Clinical Trials Services (Belgium)
Alfons G. Hoekstra, Univ. van Amsterdam (Netherlands)
Nikolay Rubtsov, Institute of Cytology and Genetics (Russian Federation)
Valerii Pavlovich Maltsev, Institute of Chemical Kinetics and Combustion (Russian Federation)

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