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Journal of Biomedical Optics

Two-photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices
Author(s): Bradley Edward Losavio; Vijay Iyer; Peter Saggau
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Paper Abstract

We developed a two-photon microscope optimized for physiologically manipulating single neurons through their postsynaptic receptors. The optical layout fulfills the stringent design criteria required for high-speed, high-resolution imaging in scattering brain tissue with minimal photodamage. We detail the practical compensation of spectral and temporal dispersion inherent in fast laser beam scanning with acousto-optic deflectors, as well as a set of biological protocols for visualizing nearly diffraction-limited structures and delivering physiological synaptic stimuli. The microscope clearly resolves dendritic spines and evokes electrophysiological transients in single neurons that are similar to endogenous responses. This system enables the study of multisynaptic integration and will assist our understanding of single neuron function and dendritic computation.

Paper Details

Date Published: 1 November 2009
PDF: 14 pages
J. Biomed. Opt. 14(6) 064033 doi: 10.1117/1.3275468
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Bradley Edward Losavio
Vijay Iyer, Howard Hughes Medical Institute (United States)
Peter Saggau, Baylor College of Medicine (United States)

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