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Journal of Biomedical Optics

Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations
Author(s): Robert N. Graf; Francisco E. Robles; Xiaoxin Chen; Adam P. Wax
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Paper Abstract

We have developed a novel dual-window approach for spectroscopic optical coherence tomography (OCT) measurements and applied it to probe nuclear morphology in tissue samples drawn from the hamster cheek pouch carcinogenesis model. The dual-window approach enables high spectral and depth resolution simultaneously, allowing detection of spectral oscillations, which we isolate to determine the structure of cell nuclei in the basal layer of the epithelium. The measurements were executed with our parallel frequency domain OCT system, which uses light from a thermal source, providing high bandwidth and access to the visible portion of the spectrum. The structural measurements show a highly statistically significant difference between untreated (normal) and treated (hyperplastic/dysplastic) tissues, indicating the potential utility of this approach as a diagnostic method.

Paper Details

Date Published: 1 November 2009
PDF: 8 pages
J. Biomed. Opt. 14(6) 064030 doi: 10.1117/1.3269680
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Robert N. Graf, Duke Univ. (United States)
Francisco E. Robles, Duke Univ. (United States)
Xiaoxin Chen, North Carolina Central Univ. (United States)
Adam P. Wax, Duke Univ. (United States)

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