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Journal of Biomedical Optics • Open Access

In vivo harmonic generation biopsy of human skin
Author(s): Szu-Yu Chen; Hai-Yin Wu; Chi-Kuang Sun

Paper Abstract

The ability to in vivo image deep tissues noninvasively with a high resolution is strongly required for optical virtual biopsy. Higher harmonic generation microscopy, combined with second- and third-harmonic generation microscopies, is applied to 17 Asian volunteers' forearm skin. After continuous observation for 30 min, no visible damage was found. Our study proves that harmonic generation biopsy (HGB) is able to satisfy the safety requirement and to provide high penetrability (~300 μm) and submicron resolution all at the same time and is a promising tool for future virtual biopsy of skin diseases. In contrast to a previous study on fixed human skin specimens, a much improved penetrability and much reduced resolution-degradation versus depth are found in this in vivo examination.

Paper Details

Date Published: 1 November 2009
PDF: 3 pages
J. Biomed. Opt. 14(6) 060505 doi: 10.1117/1.3269676
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Szu-Yu Chen, National Taiwan Univ. (Taiwan)
Hai-Yin Wu, National Taiwan Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)


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