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Journal of Biomedical Optics • Open Access

Reliability and reproducibility of macular segmentation using a custom-built optical coherence tomography retinal image analysis software
Author(s): Delia Cabrera DeBuc; Gabor Márk Somfai; Sudarshan Ranganathan; Erika Tatrai; Mária Ferencz; Carmen A. Puliafito

Paper Abstract

We determine the reliability and reproducibility of retinal thickness measurements with a custom-built OCT retinal image analysis software (OCTRIMA). Ten eyes of five healthy subjects undergo repeated standard macular thickness map scan sessions by two experienced examiners using a Stratus OCT device. Automatic/semi automatic thickness quantification of the macula and intraretinal layers is performed using OCTRIMA software. Intraobserver, interobserver, and intervisit repeatability and reproducibility coefficients, and intraclass correlation coefficients (ICCs) per scan are calculated. Intraobserver, interobserver, and intervisit variability combined account for less than 5% of total variability for the total retinal thickness measurements and less than 7% for the intraretinal layers except the outer segment/ retinal pigment epithelium (RPE) junction. There is no significant difference between scans acquired by different observers or during different visits. The ICCs obtained for the intraobserver and intervisit variability tests are greater than 0.75 for the total retina and all intraretinal layers, except the inner nuclear layer intraobserver and interobserver test and the outer plexiform layer, intraobserver, interobserver, and intervisit test. Our results indicate that thickness measurements for the total retina and all intraretinal layers (except the outer segment/RPE junction) performed using OCTRIMA are highly repeatable and reproducible.

Paper Details

Date Published: 1 November 2009
PDF: 7 pages
J. Biomed. Opt. 14(6) 064023 doi: 10.1117/1.3268773
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Delia Cabrera DeBuc, Bascom Palmer Eye Institute (United States)
Gabor Márk Somfai, Semmelweis Univ. (Hungary)
Sudarshan Ranganathan, Bascom Palmer Eye Institute (United States)
Erika Tatrai, Semmelweis Univ. (Hungary)
Mária Ferencz, Semmelweis Univ. (Hungary)
Carmen A. Puliafito, The Univ. of Southern California (United States)

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