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Journal of Biomedical Optics

Ultrafast pulse-pair control in multiphoton fluorescence laser-scanning microscopy
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Paper Abstract

In multiphoton fluorescence laser-scanning microscopy, ultrafast laser pulses [i.e., light pulses having pulse width ≤1 ps (1 ps=10-12 s)] are commonly employed to circumvent the low-multiphoton absorption cross-sections of common fluorophores. Because of the broad overlapping two-photon absorption spectra of fluorophores and the large spectral bandwidth of a short pulse, simultaneous excitation of many fluorophores is common, which justifies a persistent demand for selective excitation of individual fluorophores. We describe the use of pulse-pair excitation with possibilities of controlling molecular fluorescence in laser-scanning microscopy and compare it with coherent control using pulse sequence [De and Goswami, "Coherent control in multiphoton fluorescence imaging," Proc. SPIE 7183, 71832B (2009)].

Paper Details

Date Published: 1 November 2009
PDF: 5 pages
J. Biomed. Opt. 14(6) 064018 doi: 10.1117/1.3268440
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Arijit Kumar De, Indian Institute of Technology Kanpur (India)
Debabrata Goswami, Indian Institute of Technology Kanpur (India)


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