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Journal of Biomedical Optics • Open Access

Analysis of bacterial chemotactic response using dynamic laser speckle
Author(s): Silvia Murialdo; Gonzalo Hernán Sendra; Lucia Passoni; Ricardo Arizaga; Jorge Froilán Gonzalez; Héctor Jorge Rabal; Marcelo Trivi

Paper Abstract

Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle (or biospeckle) to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed different bacterial dynamic responses due to differences in the hardness of the support in the swarming plates. We compare this method to a conventional technique that uses white light. Both methods showed to be analogous and, in some cases, complementary. The results suggest that biospeckle processed images can be used as an alternative method to evaluate bacterial chemotactic response and can supply additional information about the bacterial motility in different areas of the swarm plate assay that might be useful for biological analysis.

Paper Details

Date Published: 1 November 2009
PDF: 6 pages
J. Biomed. Opt. 14(6) 064015 doi: 10.1117/1.3262608
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Silvia Murialdo, Univ. Nacional de Mar del Plata (Argentina)
Gonzalo Hernán Sendra, Univ. Nacional de Mar del Plata (Argentina)
Lucia Passoni, Univ. Nacional de Mar del Plata (Argentina)
Ricardo Arizaga, Univ. Nacional de la Plata (Argentina)
Jorge Froilán Gonzalez, Univ. Nacional de Mar del Plata (Argentina)
Héctor Jorge Rabal, Univ. Nacional de la Plata (Argentina)
Marcelo Trivi, Univ. Nacional de Mar del Plata (Argentina)

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