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Journal of Biomedical Optics

Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy
Author(s): Xiaofeng Zhang; Cristian Tudorel Badea; G. Allan Johnson
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Paper Abstract

We present a 3-D image reconstruction method for free-space fluorescence tomography of mice using hybrid anatomical prior information. Specifically, we use an optically reconstructed surface of the experimental animal and a digital mouse atlas to approximate the anatomy of the animal as structural priors to assist image reconstruction. Experiments are carried out on a cadaver of a nude mouse with a fluorescent inclusion (2.4-mm-diam cylinder) implanted in the chest cavity. Tomographic fluorescence images are reconstructed using an iterative algorithm based on a finite element method. Coregistration of the fluorescence reconstruction and micro-CT (computed tomography) data acquired afterward show good localization accuracy (localization error 1.2±0.6 mm). Using the optically reconstructed surface, but without the atlas anatomy, image reconstruction fails to show the fluorescent inclusion correctly. The method demonstrates the utility of anatomical priors in support of free-space fluorescence tomography.

Paper Details

Date Published: 1 November 2009
PDF: 9 pages
J. Biomed. Opt. 14(6) 064010 doi: 10.1117/1.3258836
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Xiaofeng Zhang, Ctr. for In Vivo Microscopy (United States)
Cristian Tudorel Badea, Ctr. for In Vivo Microscopy (United States)
G. Allan Johnson, Ctr. for In Vivo Microscopy (United States)

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