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Optical Engineering

Design and realization of a high-stability optical tweezer
Author(s): Slawomir Drobczynski; Pascal Hébraud; Jean-Pierre Munch; Sébastian Harlepp
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Paper Abstract

We present a method in which we stabilize mechanically an optical tweezer setup over a period ranging up to 30 min. A feedback loop is used to correct the mechanical and thermal drifts. The position of a fixed object on the sample surface is measured with a CCD device and its fluctuations analyzed and used to maintain its position fixed with three piezoelectric devices. With this setup, we obtain a spatial stability of 1.5 nm in the radial direction and 5 nm in the axial direction. This method opens the route for real-time measurements of kinetics of macromolecules association, at a single molecule level, on very long time scales.

Paper Details

Date Published: 1 November 2009
PDF: 5 pages
Opt. Eng. 48(11) 113601 doi: 10.1117/1.3257269
Published in: Optical Engineering Volume 48, Issue 11
Show Author Affiliations
Slawomir Drobczynski, Wroclaw Univ. of Technology (Poland)
Pascal Hébraud, Institut de Physique et Chimie des Matériaux de Strasbourg (France)
Jean-Pierre Munch, Institut de Physique et Chimie des Matériaux de Strasbourg (France)
Sébastian Harlepp, Institut de Physique et Chimie des Matériaux de Strasbourg (France)


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