Share Email Print

Journal of Biomedical Optics

Intracellular particle tracking as a tool for tumor cell characterization
Author(s): Yixuan Li; Jürgen Schnekenburger; Michel H. G. Duits
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We studied the dynamics of two types of intracellular probe particles, ballistically injected latex spheres and endogenous granules, in tumor cell lines of differerent metastatic potential: breast tumor cells (MCF-7 malignant, MCF-10A benign) and pancreas adenocarcinoma (PaTu8988T malignant, PaTu8988S benign). For both tissue types and for both probes, the mean squared displacement (MSD) function measured in the malignant cells was substantially larger than in the benign cells. Only a few cells were needed to characterize the tissue as malignant or benign based on their MSD, since variations in MSD within the same cell line were relatively small. These findings suggest that intracellular particle tracking (IPT) can serve as a simple and reliable method for characterization of cell states obtained from a small amount of cell sample. Mechanical analysis of the same cell lines with atomic force microscopy (AFM) in force-distance mode revealed that AFM could distinguish between the benign and malignant breast cancer cells but not the pancreatic tumor cell lines. This underlines the potential value of IPT as a complementary nanomechanical tool for studying cell-state-dependent mechanical properties.

Paper Details

Date Published: 1 November 2009
PDF: 7 pages
J. Biomed. Opt. 14(6) 064005 doi: 10.1117/1.3257253
Published in: Journal of Biomedical Optics Volume 14, Issue 6
Show Author Affiliations
Yixuan Li, Univ. Twente (Netherlands)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ Münster (Germany)
Michel H. G. Duits, Univ. Twente (Netherlands)

© SPIE. Terms of Use
Back to Top