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Journal of Biomedical Optics

Influence of laser parameters on nanoparticle-induced membrane permeabilization
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Paper Abstract

Light-absorbing nanoparticles that are heated by short laser pulses can transiently increase membrane permeability. We evaluate the membrane permeability by flow cytometry assaying of propidium iodide and fluorescein isothiocyanate dextran (FITC-D) using different laser sources. The dependence of the transfection efficiency on laser parameters such as pulse duration, irradiant exposure, and irradiation mode is investigated. For nano- and also picosecond irradiation, we show a parameter range where a reliable membrane permeabilization is achieved for 10-kDa FITC-D. Fluorescent labeled antibodies are able to penetrate living cells that are permeabilized using these parameters. More than 50% of the cells are stained positive for a 150-kDa IgG antibody. These results suggest that the laser-induced permeabilization approach constitutes a promising tool for targeted delivery of larger exogenous molecules into living cells.

Paper Details

Date Published: 1 September 2009
PDF: 7 pages
J. Biomed. Opt. 14(5) 054034 doi: 10.1117/1.3253320
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Cuiping Yao, Xi'an Jiaotong Univ. (China)
Xiaochao Qu, Xidian Univ. (China)
Zhenxi Zhang, Xi'an Jiaotong Univ. (China)
Gereon Hüttmann, Univ. zu Lübeck (Germany)
Ramtin Rahmanzadeh, Univ. zu Lübeck (Germany)

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