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Journal of Biomedical Optics • Open Access

Noninvasive assessment of testicular torsion in rabbits using frequency-domain near-infrared spectroscopy: prospects for pediatric urology
Author(s): Bertan Hallacoglu; Richard Matulewicz; Harriet J. Paltiel; Horacio Padua; Patricio Gargollo; Glenn Cannon; Ahmad Alomari; Angelo Sassaroli; Sergio Fantini

Paper Abstract

We present a quantitative near-IR spectroscopy study of the absolute values of oxygen saturation of hemoglobin before and after surgically induced testicular torsion in adult rabbits. Unilateral testicular torsions (0, 540, or 720 deg) on experimental testes and contralateral sham surgery on control testes are performed in four adult rabbits. A specially designed optical probe for measurements at multiple source-detector distances and a commercial frequency-domain tissue spectrometer are used to measure absolute values of testicular hemoglobin saturation. Our results show: (1) a consistent baseline absolute tissue hemoglobin saturation value of 78±5%, (2) a comparable tissue hemoglobin saturation of 77±6% after sham surgery, and (3) a significantly lower tissue hemoglobin saturation of 36±2% after 540- and 720-deg testicular torsion surgery. Our findings demonstrate the feasibility of performing frequency-domain, multidistance near-IR spectroscopy for absolute testicular oximetry in the assessment of testicular torsion. We conclude that near-IR spectroscopy has potential to serve as a clinical diagnostic and monitoring tool for the assessment of absolute testicular hemoglobin desaturation caused by torsion, with the possibility of serving as a complement to conventional color and spectral Doppler ultrasonography.

Paper Details

Date Published: 1 September 2009
PDF: 7 pages
J. Biomed. Opt. 14(5) 054027 doi: 10.1117/1.3253318
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Bertan Hallacoglu, Tufts Univ. (United States)
Richard Matulewicz, Tufts Univ. (United States)
Harriet J. Paltiel, Children's Hospital Boston (United States)
Horacio Padua, Children's Hospital Boston (United States)
Patricio Gargollo, Children's Hospital Boston (United States)
Glenn Cannon, Children's Hospital Boston (United States)
Ahmad Alomari, Children's Hospital Boston (United States)
Angelo Sassaroli, Tufts Univ. (United States)
Sergio Fantini, Tufts Univ. (United States)

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