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Journal of Biomedical Optics • Open Access

Specific immobilization of influenza A virus on GaAs (001) surface
Author(s): Valerie Duplan; Yannick Miron; Eric H. Frost; Michel Grandbois; Jan J. Dubowski

Paper Abstract

In the quest for the development of an all-optical biosensor for rapid detection and typing of viral pathogens, we investigate biosensing architectures that take advantage of strong photoluminescence emission from III-V quantum semiconductors (QS). One of the key elements in the development of such a biosensor is the ability to attach various analytes to GaAs-a material of choice for capping III-V QS of our interest. We report on the study of biofunctionalization of GaAs (001) with polyethylene-glycol (PEG) thiols and the successful immobilization of influenza A virus. A diluted solution of biotinylated PEG thiols in OH-terminated PEG thiols is used to form a network of sites for the attachment of neutravidin. Biotinylated polyclonal influenza A antibodies are applied to investigate the process of the immobilization of inactivated influenza A virus. The successful immobilization is demonstrated using atomic force microscopy and fluorescence microscopy measurements.

Paper Details

Date Published: 1 September 2009
PDF: 6 pages
J. Biomed. Opt. 14(5) 054042 doi: 10.1117/1.3251057
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Valerie Duplan, Univ. de Sherbrooke (Canada)
Yannick Miron, Univ. de Sherbrooke (Canada)
Eric H. Frost, Univ. de Sherbrooke (Canada)
Michel Grandbois, Univ. de Sherbrooke (Canada)
Jan J. Dubowski, Univ. de Sherbrooke (Canada)

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