Share Email Print
cover

Journal of Biomedical Optics

Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks-especially capillaries-are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.

Paper Details

Date Published: 1 September 2009
PDF: 6 pages
J. Biomed. Opt. 14(5) 054049 doi: 10.1117/1.3251044
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Song Hu, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


© SPIE. Terms of Use
Back to Top