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Journal of Biomedical Optics

Simultaneous three-dimensional optical coherence tomography and intravital microscopy for imaging subpleural pulmonary alveoli in isolated rabbit lungs
Author(s): Sven Meissner; Lilla Knels; Alexander Krueger; Thea Koch; Edmund Koch
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Paper Abstract

There is a growing interest in analyzing lung mechanics at the level of the alveoli in order to understand stress-related pathogenesis and possibly avoid ventilator associated lung injury. Emerging quantitative models to simulate fluid mechanics and the associated stresses and strains on delicate alveolar walls require realistic quantitative input on alveolar geometry and its dynamics during ventilation. Here, three-dimensional optical coherence tomography (OCT) and conventional intravital microscopy are joined in one setup to investigate the geometric changes of subpleural alveoli during stepwise pressure increase and release in an isolated and perfused rabbit lung model. We describe good qualitative agreement and quantitative correlation between the OCT data and video micrographs. Our main finding is the inflation and deflation of individual alveoli with noticeable hysteresis. Importantly, this three-dimensional geometry data can be extracted and converted into input data for numerical simulations.

Paper Details

Date Published: 1 September 2009
PDF: 6 pages
J. Biomed. Opt. 14(5) 054020 doi: 10.1117/1.3247149
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Sven Meissner, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Lilla Knels, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Alexander Krueger, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Thea Koch, Univ. Hospital Carl Gustav Carus Dresden (Germany)
Edmund Koch, Univ. Hospital Carl Gustav Carus Dresden (Germany)


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