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Journal of Biomedical Optics

Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source
Author(s): Tatsuo Kawarasaki; Kazuhiko Uchiyama; Atsushi Hirao; Sadahiro Azuma; Masayoshi Otake; Masatoshi Shibata; Seiko Tsuchiya; Shin Enosawa; Koichi Takeuchi; Kenjiro Konno; Yoji Hakamata; Hiroyuki Yoshino; Takuya Wakai; Shigeo Ookawara; Hozumi Tanaka; Eiji Kobayashi; Takashi Murakami
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Paper Abstract

Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation.

Paper Details

Date Published: 1 September 2009
PDF: 9 pages
J. Biomed. Opt. 14(5) 054017 doi: 10.1117/1.3241985
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Tatsuo Kawarasaki, Shizuoka Prefectural Livestock Institute (Japan)
Kazuhiko Uchiyama, Kitasato Univ School of Medicine (Japan)
Atsushi Hirao, Jichi Medical Univ. (Japan)
Sadahiro Azuma, Kitasato Univ School of Medicine (Japan)
Masayoshi Otake, Shizuoka Prefectural Livestock Institute (Japan)
Masatoshi Shibata, Shizuoka Prefectural Livestock Institute (Japan)
Seiko Tsuchiya, Shizuoka Prefectural Livestock Institute (Japan)
Shin Enosawa, National Research Institute for Child Health and Development (Japan)
Koichi Takeuchi, Jichi Medical Univ. (Japan)
Kenjiro Konno, Jichi Medical Univ. (Japan)
Yoji Hakamata, Jichi Medical Univ. (Japan)
Hiroyuki Yoshino, Jichi Medical Univ. (Japan)
Takuya Wakai, Jichi Medical Univ. (Japan)
Shigeo Ookawara, Jichi Medical Univ. (Japan)
Hozumi Tanaka, Jichi Medical Univ. (Japan)
Eiji Kobayashi, Jichi Medical Univ. (Japan)
Takashi Murakami, Jichi Medical Univ. (Japan)

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