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Journal of Biomedical Optics • Open Access

Live imaging of rat embryos with Doppler swept-source optical coherence tomography
Author(s): Irina V. Larina; Kenryo Furushima; Mary Elizabeth Dickinson; Richard Behringer; Kirill V. Larin

Paper Abstract

The rat has long been considered an excellent system to study mammalian embryonic cardiovascular physiology, but has lacked the extensive genetic tools available in the mouse to be able to create single gene mutations. However, the recent establishment of rat embryonic stem cell lines facilitates the generation of new models in the rat embryo to link changes in physiology with altered gene function to define the underlying mechanisms behind congenital cardiovascular birth defects. Along with the ability to create new rat genotypes there is a strong need for tools to analyze phenotypes with high spatial and temporal resolution. Doppler OCT has been previously used for 3-D structural analysis and blood flow imaging in other model species. We use Doppler swept-source OCT for live imaging of early postimplantation rat embryos. Structural imaging is used for 3-D reconstruction of embryo morphology and dynamic imaging of the beating heart and vessels, while Doppler-mode imaging is used to visualize blood flow. We demonstrate that Doppler swept-source OCT can provide essential information about the dynamics of early rat embryos and serve as a basis for a wide range of studies on functional evaluation of rat embryo physiology.

Paper Details

Date Published: 1 September 2009
PDF: 3 pages
J. Biomed. Opt. 14(5) 050506 doi: 10.1117/1.3241044
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Irina V. Larina, Baylor College of Medicine (United States)
Kenryo Furushima, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Mary Elizabeth Dickinson, Baylor College of Medicine (United States)
Richard Behringer, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Kirill V. Larin, Univ. of Houston (United States)

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