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Journal of Biomedical Optics

Virtual histology of the human heart using optical coherence tomography
Author(s): Christina M. Ambrosi; Nader Moazami; Andrew M. Rollins; Igor R. Efimov
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Paper Abstract

Optical coherence tomography (OCT) allows for the visualization of micron-scale structures within nontransparent biological tissues. For the first time, we demonstrate the use of OCT in identifying components of the cardiac conduction system and other structures in the explanted human heart. Reconstructions of cardiac structures up to 2 mm below the tissue surface were achieved and validated with Masson Trichrome histology in atrial, ventricular, sinoatrial nodal, and atrioventricular nodal preparations. The high spatial resolution of OCT provides visualization of cardiac fibers within the myocardium, as well as elements of the cardiac conduction system; however, a limiting factor remains its depth penetration, demonstrated to be ~2 mm in cardiac tissues. Despite its currently limited imaging depth, the use of OCT to identify the structural determinants of both normal and abnormal function in the intact human heart is critical in its development as a potential aid to intracardiac arrhythmia diagnosis and therapy.

Paper Details

Date Published: 1 September 2009
PDF: 7 pages
J. Biomed. Opt. 14(5) 054002 doi: 10.1117/1.3213604
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Christina M. Ambrosi, Washington Univ. in St. Louis (United States)
Nader Moazami, Washington Univ. School of Medicine in St. Louis (United States)
Andrew M. Rollins, Case Western Reserve Univ. (United States)
Igor R. Efimov, Washington Univ. in St. Louis (United States)

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