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Journal of Biomedical Optics

Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage
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Paper Abstract

We used the combination of multiphoton autofluorescence (MAF), forward second-harmonic generation (FWSHG), and backward second-harmonic generation (BWSHG) imaging for the qualitative and quantitative characterization of thermal damage of ex vivo bovine cornea. We attempt to characterize the structural alterations by qualitative MAF, FWSHG, and BWSHG imaging in the temperature range of 37 to 90°C. In addition to measuring the absolute changes in the three types of signals at the stromal surface, we also performed image correlation analysis between FWSHG and BWSHG and demonstrate that with increasing thermal damage, image correlation between FWSHG and BWSHG significantly increases. Our results show that while MAF and BWSHG intensities may be used as preliminary indicators of the extent of corneal thermal damage, the most sensitive measures are provided by the decay in FWSHG intensity and the convergence of FWSHG and BWSHG images.

Paper Details

Date Published: 1 September 2009
PDF: 6 pages
J. Biomed. Opt. 14(5) 054003 doi: 10.1117/1.3213602
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Wen Lo, National Taiwan Univ. (Taiwan)
Yu-Lin Chang, National Taiwan Univ. (Taiwan)
Jia-Shiu Liu, National Taiwan Univ. (Taiwan)
Chiu-Mei Hseuh, National Taiwan Univ. (Taiwan)
Vladimir A. Hovhannisyan, National Taiwan Univ. (Taiwan)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
Hsian-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

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