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Journal of Biomedical Optics

Spatially sculpted laser scissors for study of DNA damage and repair
Author(s): Jared P. Stephens; Samarendra Kumar Mohanty; Suzanne Genc; Xiangduo Kong; Kyoko Yokomori; Michael W. Berns
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Paper Abstract

We present a simple and efficient method for controlled linear induction of DNA damage in live cells. By passing a pulsed laser beam through a cylindrical lens prior to expansion, an elongated elliptical beam profile is created with the ability to expose controlled linear patterns while keeping the beam and the sample stationary. The length and orientation of the beam at the sample plane were reliably controlled by an adjustable aperture and rotation of the cylindrical lens, respectively. Localized immunostaining by the DNA double strand break (DSB) markers phosphorylated H2AX (γH2AX) and Nbs1 in the nuclei of HeLa cells exposed to the "line scissors" was shown via confocal imaging. The line scissors method proved more efficient than the scanning mirror and scanning stage methods at induction of DNA DSB damage with the added benefit of having a greater potential for high throughput applications.

Paper Details

Date Published: 1 September 2009
PDF: 5 pages
J. Biomed. Opt. 14(5) 054004 doi: 10.1117/1.3213601
Published in: Journal of Biomedical Optics Volume 14, Issue 5
Show Author Affiliations
Jared P. Stephens, Univ. of California, Riverside (United States)
Samarendra Kumar Mohanty, Raja Ramanna Ctr. for Advanced Technology (India)
Suzanne Genc, Beckman Laser Institute and Medical Ctr. (United States)
Xiangduo Kong, Univ. of California, Irvine (United States)
Kyoko Yokomori, Univ. of California, Irvine (United States)
Michael W. Berns, Beckman Laser Institute and Medical Ctr. (United States)

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