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Journal of Biomedical Optics

Differentiation of normal and cancerous lung tissues by multiphoton imaging
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Paper Abstract

We utilize multiphoton microscopy for the label-free diagnosis of noncancerous, lung adenocarcinoma (LAC), and lung squamous cell carcinoma (SCC) tissues from humans. Our results show that the combination of second-harmonic generation (SHG) and multiphoton excited autofluorescence (MAF) signals may be used to acquire morphological and quantitative information in discriminating cancerous from noncancerous lung tissues. Specifically, noncancerous lung tissues are largely fibrotic in structure, while cancerous specimens are composed primarily of tumor masses. Quantitative ratiometric analysis using MAF to SHG index (MAFSI) shows that the average MAFSI for noncancerous and LAC lung tissue pairs are 0.55±0.23 and 0.87±0.15, respectively. In comparison, the MAFSIs for the noncancerous and SCC tissue pairs are 0.50±0.12 and 0.72±0.13, respectively. Our study shows that nonlinear optical microscopy can assist in differentiating and diagnosing pulmonary cancer from noncancerous tissues.

Paper Details

Date Published: 1 July 2009
PDF: 4 pages
J. Biomed. Opt. 14(4) 044034 doi: 10.1117/1.3210768
Published in: Journal of Biomedical Optics Volume 14, Issue 4
Show Author Affiliations
Chun-Chin Wang, National Taiwan Univ. (Taiwan)
Feng-Chieh Li, National Taiwan Univ. (Taiwan)
Ruei-Jr Wu, National Taiwan Univ. (Taiwan)
Vladimir A. Hovhannisyan, National Taiwan Univ. (Taiwan)
Wei-Chou Lin, National Taiwan Univ. Hospital (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


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