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Journal of Biomedical Optics • Open Access

Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes
Author(s): Hieu M. Dang; Gen Omura; Toshiyuki Omano; Masatomo Yamagiwa; Shin'ichiro Kajiyama; Yasuyuki Ozeki; Kazuyoshi Itoh; Kiichi Fukui

Paper Abstract

We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized.

Paper Details

Date Published: 1 July 2009
PDF: 3 pages
J. Biomed. Opt. 14(4) 040506 doi: 10.1117/1.3207151
Published in: Journal of Biomedical Optics Volume 14, Issue 4
Show Author Affiliations
Hieu M. Dang, Osaka Univ. (Japan)
Gen Omura, Osaka Univ. (Japan)
Toshiyuki Omano, Osaka Univ. (Japan)
Masatomo Yamagiwa, Osaka Univ. (Japan)
Shin'ichiro Kajiyama, Osaka Univ. (Japan)
Yasuyuki Ozeki, Osaka Univ. (Japan)
Kazuyoshi Itoh, Osaka Univ. (Japan)
Kiichi Fukui, Osaka Univ. (Japan)

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