Share Email Print
cover

Journal of Biomedical Optics

Three-dimensional cardiac architecture determined by two-photon microtomy
Author(s): Hayden Huang; Catherine MacGillivray; Hyuk-Sang Kwon; Jan Lammerding; Jeffrey Robbins; Richard T. Lee; Peter T. C. So
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Cardiac architecture is inherently three-dimensional, yet most characterizations rely on two-dimensional histological slices or dissociated cells, which remove the native geometry of the heart. We previously developed a method for labeling intact heart sections without dissociation and imaging large volumes while preserving their three-dimensional structure. We further refine this method to permit quantitative analysis of imaged sections. After data acquisition, these sections are assembled using image-processing tools, and qualitative and quantitative information is extracted. By examining the reconstructed cardiac blocks, one can observe end-to-end adjacent cardiac myocytes (cardiac strands) changing cross-sectional geometries, merging and separating from other strands. Quantitatively, representative cross-sectional areas typically used for determining hypertrophy omit the three-dimensional component; we show that taking orientation into account can significantly alter the analysis. Using fast-Fourier transform analysis, we analyze the gross organization of cardiac strands in three dimensions. By characterizing cardiac structure in three dimensions, we are able to determine that the α crystallin mutation leads to hypertrophy with cross-sectional area increases, but not necessarily via changes in fiber orientation distribution.

Paper Details

Date Published: 1 July 2009
PDF: 10 pages
J. Biomed. Opt. 14(4) 044029 doi: 10.1117/1.3200939
Published in: Journal of Biomedical Optics Volume 14, Issue 4
Show Author Affiliations
Hayden Huang, Columbia Univ. (United States)
Catherine MacGillivray, Brigham and Women's Hospital (United States)
Hyuk-Sang Kwon, Massachusetts Institute of Technology (United States)
Jan Lammerding, Brigham and Women's Hospital (United States)
Jeffrey Robbins, Cincinnati Children's Hospital Medical Ctr. (United States)
Richard T. Lee, Brigham and Women's Hospital (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)


© SPIE. Terms of Use
Back to Top