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Journal of Biomedical Optics

Broadband diffuse optical spectroscopy assessment of hemorrhage- and hemoglobin-based blood substitute resuscitation
Author(s): Jangwoen Lee; Jae Gwan Kim; Sari B. Mahon; Bruce Jason Tromberg; David Sho Mukai; Kelly A. Kreuter; Darin J. Saltzman; Renee Patino; Robert Goldberg; Matthew Brenner
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Paper Abstract

Hemoglobin-based oxygen carriers (HBOCs) are solutions of cell-free hemoglobin (Hb) that have been developed for replacement or augmentation of blood transfusion. It is important to monitor in vivo tissue hemoglobin content, total tissue hemoglobin [THb], oxy- and deoxy-hemoglobin concentrations ([OHb], [RHb]), and tissue oxygen saturation (StO2=[OHb]/[THb]×100%) to evaluate effectiveness of HBOC transfusion. We designed and constructed a broadband diffuse optical spectroscopy (DOS) prototype system to measure bulk tissue absorption and scattering spectra between 650 and 1000 nm capable of accurately determining these tissue hemoglobin component concentrations in vivo. Our purpose was to assess the feasibility of using DOS to optically monitor tissue [OHb], [RHb], StO2, and total tissue hemoglobin concentration ([THb]=[OHb]+[RHb]) during HBOC infusion using a rabbit hypovolemic shock model. The DOS prototype probe was placed on the shaved inner thigh muscle of the hind leg to assess concentrations of [OHb], [RHb], [THb], as well as StO2. Hemorrhagic shock was induced in intubated New Zealand white rabbits (N=6) by withdrawing blood via a femoral arterial line to 20% blood loss (10-15 cc/kg). Hemoglobin glutamer-200 (Hb-200) 1:1 volume resuscitation was administered following the hemorrhage. These values were compared against traditional invasive measurements, serum hemoglobin concentration (sHGB), systemic blood pressure, heart rate, and blood gases. DOS revealed increases of [THb], [OHb], and tissue hemoglobin oxygen saturation after Hb-200 infusion, while blood total hemoglobin values continued did not increase; we speculate, due to hyperosmolality induced hemodilution. DOS enables noninvasive in vivo monitoring of tissue hemoglobin and oxygenation parameters during shock and volume expansion with HBOC and potentially enables the assessment of efficacy of resuscitation efforts using artificial blood substitutes.

Paper Details

Date Published: 1 July 2009
PDF: 7 pages
J. Biomed. Opt. 14(4) 044027 doi: 10.1117/1.3200932
Published in: Journal of Biomedical Optics Volume 14, Issue 4
Show Author Affiliations
Jangwoen Lee, Beckman Laser Institute and Medical Ctr. (United States)
Jae Gwan Kim, Beckman Laser Institute and Medical Ctr. (United States)
Sari B. Mahon, Beckman Laser Institute and Medical Ctr. (United States)
Bruce Jason Tromberg, Beckman Laser Institute and Medical Ctr. (United States)
David Sho Mukai, Beckman Laser Institute and Medical Ctr. (United States)
Kelly A. Kreuter, Beckman Laser Institute and Medical Ctr. (United States)
Darin J. Saltzman, Beckman Laser Institute and Medical Ctr. (United States)
Renee Patino, Univ. of California, Irvine (United States)
Robert Goldberg, Univ. of California, Irvine (United States)
Matthew Brenner, Univ. of California, Irvine (United States)

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