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Journal of Biomedical Optics • Open Access

Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy
Author(s): Dean C. S. Tai; Nancy Tan; Shuoyu Xu; Chiang Huen Kang; Ser Mien Chia; Chee Leong Cheng; Aileen Wee; Chiang Li Wei; Anju Mythereyi Raja; Guangfa Xiao; Shi Chang; Jagath C. Rajapakse; Peter T. C. So; Hui-Huan Tang; Chien Shing Chen; Hanary Yu

Paper Abstract

We develop a standardized, fully automated, quantification system for liver fibrosis assessment using second harmonic generation microscopy and a morphology-based quantification algorithm. Liver fibrosis is associated with an abnormal increase in collagen as a result of chronic liver diseases. Histopathological scoring is the most commonly used method for liver fibrosis assessment, where a liver biopsy is stained and scored by experienced pathologists. Due to the intrinsic limited sensitivity and operator-dependent variations, there exist high inter- and intraobserver discrepancies. We validate our quantification system, Fibro-C-Index, with a comprehensive animal study and demonstrate its potential application in clinical diagnosis to reduce inter- and intraobserver discrepancies.

Paper Details

Date Published: 1 July 2009
PDF: 10 pages
J. Biomed. Opt. 14(4) 044013 doi: 10.1117/1.3183811
Published in: Journal of Biomedical Optics Volume 14, Issue 4
Show Author Affiliations
Dean C. S. Tai, Institute of Bioengineering and Nanotechnology (Singapore)
Nancy Tan, National Univ. of Singapore (Singapore)
Shuoyu Xu, Institute of Bioengineering and Nanotechnology (Singapore)
Chiang Huen Kang, Institute of Bioengineering and Nanotechnology (Singapore)
Ser Mien Chia, National Univ. of Singapore (Singapore)
Chee Leong Cheng, National Univ. Hospital (Singapore)
Aileen Wee, National Univ. Hospital (Singapore)
Chiang Li Wei, KK Women's and Children's Hospital (Singapore)
Anju Mythereyi Raja, Institute of Bioengineering and Nanotechnology (Singapore)
Guangfa Xiao, Xiangya Hospital of Central South Univ. (China)
Shi Chang, Xiangya Hospital of Central South Univ. (China)
Jagath C. Rajapakse, Nanyang Technological Univ. (Singapore)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Hui-Huan Tang, Xiangya Hospital of Central South Univ. (China)
Chien Shing Chen, National Univ. of Singapore (Singapore)
Hanary Yu, National Univ. of Singapore (Singapore)

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