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Journal of Biomedical Optics • Open Access

Probing cell surface interactions using atomic force microscope cantilevers functionalized for quantum dot-enabled Förster resonance energy transfer
Author(s): Zhe Sun; Ameet Juriani; Gerald A. Meininger; Kenith E. Meissner

Paper Abstract

Förster resonance energy transfer (FRET) between quantum dot (QD) donors and red fluorescent protein (RFP)-tagged integrin acceptors in live cells is reported for the first time. A silica microsphere was coated with CdSe/ZnS QDs and mounted to the cantilever of an atomic force microscope (AFM). The QD microsphere is then conjugated with fibronectin to bind with RFP-αv integrins expressed on the surface of HeLa cells. Following AFM-controlled cell contact with the QD-microsphere structure, FRET is observed between the QD-RFP pair using a photobleaching measurement technique. This FRET probe technique provides a novel tool for studying the cell surface receptor-ligand interactions in biomedical and biological research.

Paper Details

Date Published: 1 July 2009
PDF: 3 pages
J. Biomed. Opt. 14(4) 040502 doi: 10.1117/1.3174429
Published in: Journal of Biomedical Optics Volume 14, Issue 4
Show Author Affiliations
Zhe Sun, Univ. of Missouri, Columbia (United States)
Ameet Juriani, Texas A&M Univ. (United States)
Gerald A. Meininger, Univ. of Missouri, Columbia (United States)
Kenith E. Meissner, Texas A&M Univ. (United States)

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