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Journal of Micro/Nanolithography, MEMS, and MOEMS

Obstacles to the production of protein microarray cassettes
Author(s): Jean I. Montagu; Herman DeWeerd; Nathan Tyburczy; Natalia Rodionova; Peter Maimonis
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Paper Abstract

We examine the necessary technologies to be mastered in order to build a practical microarray-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface chemistry, dye stability, and imaging are outlined, showing why a treated 100-nm film of nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined, and a practical solution proposed. Wet and dry imaging are compared. The steps and functions expected for an assay platform comprising a processing station and biochip cassette are identified. The performance of a successful benchtop automated multiplex immunoassay system is briefly described.

Paper Details

Date Published: 1 July 2009
PDF: 7 pages
J. Micro/Nanolith. MEMS MOEMS 8(3) 033013 doi: 10.1117/1.3158071
Published in: Journal of Micro/Nanolithography, MEMS, and MOEMS Volume 8, Issue 3
Show Author Affiliations
Jean I. Montagu, Decision Biomarkers, Inc. (United States)
Herman DeWeerd, Decision Biomarkers, Inc. (United States)
Nathan Tyburczy, Decision Biomarkers, Inc. (United States)
Natalia Rodionova, Decision Biomarkers, Inc. (United States)
Peter Maimonis, Decision Biomarkers, Inc. (United States)

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