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Journal of Biomedical Optics

Acoustic fingerprints of dye-labeled protein submicrosphere photoacoustic contrast agents
Author(s): Michael A. McDonald; Ladislav Jankovic; Khalid Shahzad; Michael Burcher; King C. P. Li
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Paper Abstract

Dye-labeled protein microspheres, submicron in size and capable of producing thermoelastically generated ultrasound in response to laser stimulation, are presented as contrast agents for photoacoustic imaging. Incident laser energy absorbed by fluorescein isothiocyanate (FITC)-labeled elastin submicrospheres results in thermoelastically generated sound production. Plotted A-line graphs reveal a distinctive morphology and a greater than two orders of magnitude increase in signal amplitude subsequent to converting FITC elastin into submicrospheres (despite a four orders of magnitude decrease in concentration). Evidence of nonlinearity and enhancement of ultrasound backscatter indicate a potential use in contrast-enhanced harmonic imaging. Photoacoustic and ultrasound imaging of FITC-elastin submicrospheres in a water-filled phantom vessel shows enhanced contrast at low concentration and clear delineation of the phantom vessel wall.

Paper Details

Date Published: 1 May 2009
PDF: 7 pages
J. Biomed. Opt. 14(3) 034032 doi: 10.1117/1.3155519
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Michael A. McDonald, National Institute of Standards and Technology (United States)
Ladislav Jankovic, Philips Research (United States)
Khalid Shahzad, Philips Research (United States)
Michael Burcher, Philips Research (United States)
King C. P. Li, Methodist Hospital Research Institute (United States)

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