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Journal of Biomedical Optics • Open Access

High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells
Author(s): Céline C. B. Boutin; Yann Roche; Christine Millot; Regis Deturche; Pascal Royer; Michel Manfait; Jérôme Plain; Pierre Jeannesson; Jean-Marc Millot; Rodolphe Jaffiol

Paper Abstract

Diffusion-time distribution analysis (DDA) has been used to explore the plasma membrane fluidity of multidrug-resistant cancer cells (LR73 carcinoma cells) and also to characterize the influence of various membrane agents present in the extracellular medium. DDA is a recent single-molecule technique, based on fluorescence correlation spectroscopy (FCS), well suited to retrieve local organization of cell membrane. The method was conducted on a large number of living cells, which enabled us to get a detailed overview of plasma membrane microviscosity, and plasma membrane micro-organization, between the cells of the same line. Thus, we clearly reveal the higher heterogeneity of plasma membrane in multidrug-resistant cancer cells in comparison with the nonresistant ones (denoted sensitive cells). We also display distinct modifications related to a membrane fluidity modulator, benzyl alcohol, and two revertants of multidrug resistance, verapamil and cyclosporin-A. A relation between the distribution of the diffusion-time values and the modification of membrane lateral heterogeneities is proposed.

Paper Details

Date Published: 1 May 2009
PDF: 8 pages
J. Biomed. Opt. 14(3) 034030 doi: 10.1117/1.3155518
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Céline C. B. Boutin, Univ. de Technologie Troyes (France)
Yann Roche, Univ. de Reims Champagne-Ardenne (France)
Christine Millot, Univ. de Reims Champagne-Ardenne (France)
Regis Deturche, Univ. de Technologie Troyes (France)
Pascal Royer, Univ. de Technologie Troyes (France)
Michel Manfait, Univ. de Reims Champagne-Ardenne (France)
Jérôme Plain, Univ. de Technologie Troyes (France)
Pierre Jeannesson, Univ. de Reims Champagne-Ardenne (France)
Jean-Marc Millot, Univ. de Reims Champagne-Ardenne (France)
Rodolphe Jaffiol, Univ. de Technologie Troyes (France)

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