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Journal of Biomedical Optics

In-vivo Fourier domain optical coherence tomography as a new tool for investigation of vasodynamics in the mouse model
Author(s): Sven Meissner; Gregor Müller; Julia Walther; Henning Morawietz; Edmund Koch
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Paper Abstract

In-vivo imaging of the vascular system can provide novel insight into the dynamics of vasoconstriction and vasodilation. Fourier domain optical coherence tomography (FD-OCT) is an optical, noncontact imaging technique based on interferometry of short-coherent near-infrared light with axial resolution of less than 10 μm. In this study, we apply FD-OCT as an in-vivo imaging technique to investigate blood vessels in their anatomical context using temporally resolved image stacks. Our chosen model system is the murine saphenous artery and vein, due to their small inner vessel diameters, sensitive response to vasoactive stimuli, and advantageous anatomical position. The vascular function of male wild-type mice (C57BL/6) is determined at the ages of 6 and 20 weeks. Vasoconstriction is analyzed in response to dermal application of potassium (K+), and vasodilation in response to sodium nitroprusside (SNP). Vasodynamics are quantified from time series (75 sec, 4 frames per sec, 330×512 pixels per frame) of cross sectional images that are analyzed by semiautomated image processing software. The morphology of the saphenous artery and vein is determined by 3-D image stacks of 512×512×512 pixels. Using the FD-OCT technique, we are able to demonstrate age-dependent differences in vascular function and vasodynamics.

Paper Details

Date Published: 1 May 2009
PDF: 6 pages
J. Biomed. Opt. 14(3) 034027 doi: 10.1117/1.3149865
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Sven Meissner, Technische Univ. Dresden (Germany)
Gregor Müller, Technische Univ. Dresden (Germany)
Julia Walther, Technische Univ. Dresden (Germany)
Henning Morawietz, Technische Univ. Dresden (Germany)
Edmund Koch, Technische Univ. Dresden (Germany)

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