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Journal of Biomedical Optics • Open Access

Fabrication of indocyanine green encapsulated biodegradable microbubbles for structural and functional imaging of cancer
Author(s): Ronald X. Xu; Jiwei Huang; Jeff S. Xu; Duxin Sun; George H. Hinkle; Edward W. M. Martin; Stephen P. Povoski

Paper Abstract

We developed a novel dual-modal contrast agent for the structural and functional imaging of cancer. The contrast agent was fabricated by encapsulating indocyanine green (ICG) in poly(lactic-co-glycolic acid) (PLGA) microbubbles using a modified double-emulsion method. More stabilized absorption and fluorescence emission characteristics were observed for aqueous and plasma suspensions of ICG-encapsulated microbubbles. The technical feasibility of concurrent structural and functional imaging was demonstrated through a series of benchtop tests in which the aqueous suspension of ICG-encapsulated microbubbles was injected into a transparent tube embedded in an Intralipid phantom at different flow rates and concentrations. Concurrent fluorescence imaging and B-mode ultrasound imaging successfully captured the changes of microbubble flow rate and concentration with high linearity and accuracy. One potential application of the proposed ICG-encapsulated PLGA microbubbles is for the identification and characterization of peritumoral neovasculature for enhanced coregistration between tumor structural and functional boundaries in ultrasound-guided near-infrared diffuse optical tomography.

Paper Details

Date Published: 1 May 2009
PDF: 6 pages
J. Biomed. Opt. 14(3) 034020 doi: 10.1117/1.3147424
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Ronald X. Xu, The Ohio State Univ. (United States)
Jiwei Huang, The Ohio State Univ. (United States)
Jeff S. Xu, The Ohio State Univ. (United States)
Duxin Sun, The Ohio State Univ. (United States)
George H. Hinkle, The Ohio State Univ. (United States)
Edward W. M. Martin, The Ohio State Univ. (United States)
Stephen P. Povoski, The Ohio State Univ. (United States)

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