Share Email Print
cover

Journal of Biomedical Optics

Dual-spectrum laser source based on fiber continuum generation for integrated optical coherence and multiphoton microscopy
Author(s): Benedikt W. Graf; Zhi Jiang; Haohua Tu; Stephen A. Boppart
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

A single-laser dual-spectrum source designed for integrated optical coherence and multiphoton microscopy is demonstrated. The source implements the laser characteristics needed to optimally perform both modalities while extending the spectral range for this imaging technique. It consists of a widely tunable, mode-locked, Ti-sapphire laser with a portion of its output spectrally broadened via continuum generation in a photonic crystal fiber. The continuum-broadened beam allows for enhanced optical sectioning with optical coherence microscopy, while the unbroadened beam from the ultrashort-pulse Ti-sapphire laser optimally excites fluorescent markers. The noise power of the continuum-broadened beam is less than 1.1 dBm/Hz higher than the Ti-sapphire laser in the range from 1 Hz to 25 MHz, and the fiber shows no sign of damage after ~100 h of use. We demonstrate the use of this source across a wide spectral range by imaging green fluorescent protein-transfected mouse fibroblast cells costained with fluorescent dyes that are maximally excited at various wavelengths. Images of unstained in vivo human skin are also presented. This source extends the feasibility of this integrated imaging modality and will facilitate new investigations in in vivo microscopy, tissue engineering, and cell biology.

Paper Details

Date Published: 1 May 2009
PDF: 6 pages
J. Biomed. Opt. 14(3) 034019 doi: 10.1117/1.3147422
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Benedikt W. Graf, Univ. of Illinois at Urbana-Champaign (United States)
Zhi Jiang, Univ. of Illinois at Urbana-Champaign (United States)
Haohua Tu, Univ. of Illinois at Urbana-Champaign (United States)
Stephen A. Boppart, Univ. of Illinois at Urbana-Champaign (United States)


© SPIE. Terms of Use
Back to Top