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Journal of Biomedical Optics

Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy
Author(s): Chia-Wei Lee; Miin-Jang Chen; Ji-Yen Cheng; Pei-Kuen Wei
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Paper Abstract

The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times.

Paper Details

Date Published: 1 May 2009
PDF: 6 pages
J. Biomed. Opt. 14(3) 034016 doi: 10.1117/1.3147390
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Chia-Wei Lee, Research Ctr. for Applied Sciences (Taiwan)
Miin-Jang Chen, National Taiwan Univ. (Taiwan)
Ji-Yen Cheng, Research Ctr. for Applied Sciences (Taiwan)
Pei-Kuen Wei, Research Ctr. for Applied Sciences (Taiwan)


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