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Journal of Biomedical Optics

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy
Author(s): Benjamin Rappaz; Elena Cano; Tristan Colomb; Jonas Kuhn; Christian D. Depeursinge; Viesturs Simanis; Pierre J. Magistretti; Pierre P. Marquet
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Paper Abstract

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.

Paper Details

Date Published: 1 May 2009
PDF: 5 pages
J. Biomed. Opt. 14(3) 034049 doi: 10.1117/1.3147385
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Benjamin Rappaz, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Elena Cano, Swiss Institute for Experimental Cancer Research (Switzerland)
Tristan Colomb, Lyncée Tec SA (Switzerland)
Jonas Kuhn, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christian D. Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Viesturs Simanis, Swiss Institute for Experimental Cancer Research (Switzerland)
Pierre J. Magistretti, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Pierre P. Marquet, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


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