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Journal of Biomedical Optics

Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging
Author(s): James J. Mancuso; Adam M. Larson; Theodore G. Wensel; Peter Saggau
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Paper Abstract

The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

Paper Details

Date Published: 1 May 2009
PDF: 5 pages
J. Biomed. Opt. 14(3) 034048 doi: 10.1117/1.3139850
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
James J. Mancuso, Baylor College of Medicine (United States)
Adam M. Larson, Baylor College of Medicine (United States)
Theodore G. Wensel, Baylor College of Medicine (United States)
Peter Saggau, Baylor College of Medicine (United States)

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