Share Email Print

Journal of Biomedical Optics

Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds
Author(s): Yali Jia; Pierre-Olivier Bagnaninchi; Ying Yang; Alicia J. El Haj; Monica T. Hinds; Sean J. Kirkpatrick; Ruikang Wang
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Establishing a relationship between perfusion rate and fluid shear stress in a 3D cell culture environment is an ongoing and challenging task faced by tissue engineers. We explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low- and high-porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5 ml·min-1. The DOCT results show that the behavior of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low-porosity and high-porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore, with a mean shear stress of 0.49±0.3 dyn·cm-2 and 0.38±0.2 dyn·cm-2, respectively. In addition, we show that the scaffold's porosity and interconnectivity can be quantified by combining analyses of the 3D structural and flow images obtained from DOCT.

Paper Details

Date Published: 1 May 2009
PDF: 9 pages
J. Biomed. Opt. 14(3) 034014 doi: 10.1117/1.3130345
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Yali Jia, Oregon Health & Science Univ. (United States)
Pierre-Olivier Bagnaninchi, Univ. of Edinburgh (United Kingdom)
Ying Yang, Keele Univ. (United Kingdom)
Alicia J. El Haj, Keele Univ. (United Kingdom)
Monica T. Hinds, Oregon Health & Science Univ. (United States)
Sean J. Kirkpatrick, Oregon Health & Science Univ. (United States)
Ruikang Wang, Oregon Health & Science Univ. (United States)

© SPIE. Terms of Use
Back to Top