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Journal of Biomedical Optics • Open Access

Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle
Author(s): Silvia Santos; Kengyeh K. Chu; Daryl Lim; Nenad Bozinovic; Timothy N. Ford; Claire Hourtoule; Aaron C. Bartoo; Satish K. Singh; Jerome Mertz

Paper Abstract

We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an optically sectioned image with low resolution. This is complemented with high-resolution information from the uniform illumination image, leading to a full-resolution image that is optically sectioned. HiLo endomicroscope movies are presented of fluorescently labeled rat colonic mucosa.

Paper Details

Date Published: 1 May 2009
PDF: 3 pages
J. Biomed. Opt. 14(3) 030502 doi: 10.1117/1.3130266
Published in: Journal of Biomedical Optics Volume 14, Issue 3
Show Author Affiliations
Silvia Santos, Boston Univ. (United States)
Kengyeh K. Chu, Boston Univ. (United States)
Daryl Lim, Boston Univ. (United States)
Nenad Bozinovic, Boston Univ. (United States)
Timothy N. Ford, Boston Univ. (United States)
Claire Hourtoule, Boston Univ. (United States)
Aaron C. Bartoo, Boston Univ. School of Medicine (United States)
Satish K. Singh, Boston Univ. School of Medicine (United States)
Jerome Mertz, Boston Univ. (United States)

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