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Journal of Biomedical Optics

Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment
Author(s): Tatiana A. Zdobnova; S. G. Dorofeev; P. N. Tananaev; R. B. Vasiliev; T. G. Balandin; Eveline F. Edelweiss; O. A. Stremovskiy; Irina V. Balalaeva; Ilya V. Turchin; Elena N. Lebedenko; Vladimir P. Zlomanov; Sergey M. Deyev
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Paper Abstract

Semiconductor quantum dots (QDs) coupled with cancer-specific targeting ligands are new promising agents for fluorescent visualization of cancer cells. Human epidermal growth factor receptor 2/neu (HER2/neu), overexpressed on the surface of many cancer cells, is an important target for cancer diagnostics. Antibody scFv fragments as a targeting agent for direct delivery of fluorophores offer significant advantages over full-size antibodies due to their small size, lower cross-reactivity, and immunogenicity. We have used quantum dots linked to anti-HER2/neu 4D5 scFv antibody to label HER2/neu-overexpressing live cells. Labeling of target cells was shown to have high brightness, photostability, and specificity. The results indicate that construction based on quantum dots and scFv antibody can be successfully used for cancer cell visualization.

Paper Details

Date Published: 1 March 2009
PDF: 5 pages
J. Biomed. Opt. 14(2) 021004 doi: 10.1117/1.3122775
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Tatiana A. Zdobnova, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
S. G. Dorofeev, Lomonosov Moscow State Univ. (Russian Federation)
P. N. Tananaev, Lomonosov Moscow State Univ. (Russian Federation)
R. B. Vasiliev, Lomonosov Moscow State Univ. (Russian Federation)
T. G. Balandin, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Eveline F. Edelweiss, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
O. A. Stremovskiy, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Irina V. Balalaeva, Nizhny Novgorod State Univ. (Russian Federation)
Ilya V. Turchin, Institute of Applied Physics (Russian Federation)
Elena N. Lebedenko, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Vladimir P. Zlomanov, Lomonosov Moscow State Univ. (Russian Federation)
Sergey M. Deyev, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)


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