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Journal of Biomedical Optics • Open Access

Monte Carlo modeling of multilayer phantoms with multiple fluorophores: simulation algorithm and experimental validation

Paper Abstract

This work is first a description of a statistical simulation algorithm developed for simulating the spectral absorption and emission of several fluorophores in an absorbing and diffusing multilayer model. Second, a detailed experimental validation of the simulation program is conducted on two sets of liquid and solid multilayer phantoms, containing one, two, or three fluorophores, within absorbing and scattering media. Experimental spatially resolved reflectance spectra are acquired in the wavelength band 400 to 800 nm and compared to corresponding simulated spectra. The degree of similarity between experimentation and simulation data is quantified. The results obtained underline good correlations with mean errors varying from 2 to 10%, depending on the number of layers and on the complexity of the phantom's composition.

Paper Details

Date Published: 1 March 2009
PDF: 11 pages
J. Biomed. Opt. 14(2) 024048 doi: 10.1117/1.3122368
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Emilie Pery, Ctr. de Recherche en Automatique de Nancy (France)
Walter C. P. M. Blondel, CRAN INPL (France)
Cedric Thomas, Ctr. de Recherche en Automatique de Nancy (France)
François H. Guillemin, Ctr. de Recherche en Automatique de Nancy (France)

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