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Journal of Biomedical Optics

Registration of planar bioluminescence to magnetic resonance and x-ray computed tomography images as a platform for the development of bioluminescence tomography reconstruction algorithms
Author(s): Bradley J. Beattie; Alexander D. Klose; Carl H. Le; Valerie A. Longo; Konstantine Dobrenkov; Jelena Vider; Jason A. Koutcher; Ronald G. Blasberg
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Paper Abstract

The procedures we propose make possible the mapping of two-dimensional (2-D) bioluminescence image (BLI) data onto a skin surface derived from a three-dimensional (3-D) anatomical modality [magnetic resonance (MR) or computed tomography (CT)] dataset. This mapping allows anatomical information to be incorporated into bioluminescence tomography (BLT) reconstruction procedures and, when applied using sources visible to both optical and anatomical modalities, can be used to evaluate the accuracy of those reconstructions. Our procedures, based on immobilization of the animal and a priori determined fixed projective transforms, should be more robust and accurate than previously described efforts, which rely on a poorly constrained retrospectively determined warping of the 3-D anatomical information. Experiments conducted to measure the accuracy of the proposed registration procedure found it to have a mean error of 0.36±0.23 mm. Additional experiments highlight some of the confounds that are often overlooked in the BLT reconstruction process, and for two of these confounds, simple corrections are proposed.

Paper Details

Date Published: 1 March 2009
PDF: 9 pages
J. Biomed. Opt. 14(2) 024045 doi: 10.1117/1.3120495
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Bradley J. Beattie, Memorial Sloan-Kettering Cancer Ctr. (United States)
Alexander D. Klose, Columbia Univ. (United States)
Carl H. Le, Memorial Sloan-Kettering Cancer Ctr. (United States)
Valerie A. Longo, Memorial Sloan-Kettering Cancer Ctr. (United States)
Konstantine Dobrenkov, Memorial Sloan-Kettering Cancer Ctr. (United States)
Jelena Vider, Memorial Sloan-Kettering Cancer Ctr. (United States)
Jason A. Koutcher, Memorial Sloan-Kettering Cancer Ctr. (United States)
Ronald G. Blasberg, Memorial Sloan-Kettering Cancer Ctr. (United States)


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