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Journal of Biomedical Optics

Characterizing liquid turbid media by frequency-domain photon-migration spectroscopy
Author(s): Biju Cletus; Rainer Kunnemeyer; Paul Martinsen; Andrew McGlone; Robert Jordan
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Paper Abstract

We present a wavelength-tunable frequency-domain instrument for the characterization of liquid turbid media. The instrument employs a tunable titanium-sapphire laser modulated by an acousto-optic modulator. The absorption and reduced scattering coefficient of Intralipid® 20%, diluted to concentrations of 0.94 to 4.00%, are measured over the wavelength range 710 to 850 nm at 10-nm intervals. The standard measurement errors for the absorption and reduced scattering coefficients are 1 and 2.5%, respectively. Extrapolation to 0% Intralipid® concentration gives an absorption coefficient that closely follows that of water, overestimating the absorption of pure water by less than 10%. The reduced scattering coefficient is compared at 750 nm with published results and is found consistent within the experimental error. We compare the reduced scattering coefficient to an estimate based on Mie theory and find the reduced scattering coefficient underestimated the Mie theory result by about 9%.

Paper Details

Date Published: 1 March 2009
PDF: 7 pages
J. Biomed. Opt. 14(2) 024041 doi: 10.1117/1.3119282
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Biju Cletus, Plant & Food Research (New Zealand)
Rainer Kunnemeyer, The Univ. of Waikato (New Zealand)
Paul Martinsen, Plant & Food Research (New Zealand)
Andrew McGlone, Hort Research (New Zealand)
Robert Jordan, Hort Research (New Zealand)

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