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Journal of Biomedical Optics • Open Access

Study of gold nanoparticles and live cells interactions by using planar evanescent wave excitation
Author(s): Chia-Wei Lee; En-Hong Lin; Ji-Yen Cheng; Pei-Kuen Wei

Paper Abstract

We present a planar evanescent wave (PEW) technique combined with phase contrast optical microscopy to study the interactions between cells and gold nanoparticles (AuNPs). The PEW method employs a dual-fiber-line guide to couple light into a thin glass slide. It produces a uniform and long evanescent wave near the glass surface, as verified by the optical near-field measurement. High-contrast AuNP images are obtained by the PEW illumination. At the same time, cells are observed only by using the phase contrast microscopy. The nanoparticles and cell images indicated that unmodified AuNPs had no interactions with cells, possibly due to the negative surface charges on both cells and nanoparticles. The electrostatic concept was further verified by coating AuNPs with positively charged poly (L-lysine). DNA aptamers for surface mucin glycoprotein were coated on AuNPs to demonstrate the application for single nanoparticle tracking.

Paper Details

Date Published: 1 March 2009
PDF: 6 pages
J. Biomed. Opt. 14(2) 021005 doi: 10.1117/1.3116710
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Chia-Wei Lee, Research Ctr. for Applied Sciences (Taiwan)
En-Hong Lin, Research Ctr. for Applied Sciences (Taiwan)
Ji-Yen Cheng, Research Ctr. for Applied Sciences (Taiwan)
Pei-Kuen Wei, Research Ctr. for Applied Sciences (Taiwan)

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