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Journal of Biomedical Optics

Effects of Er:YAG laser irradiation and manipulation treatments on dentin components, part 2: energy-dispersive X-ray fluorescence spectrometry study
Author(s): Luís Eduardo Silva Soares; Ana Maria Do Espirito Santo; Aldo Brugnera; Fátima Antonia Aparecida Zanin; Airton Abrahao Martin
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Paper Abstract

The effects of laser etching, decontamination, and storage treatments on dentin components were studied by energy-dispersive X-ray fluorescence spectrometry (EDXRF). Thirty bovine incisors were prepared to expose the dentin surface and then divided into two main groups based upon the decontamination process and storage procedure: autoclaved (group A, n=15) or stored in aqueous thymol solution (group B, n=15). The surfaces of the dentin slices were schematically divided into four areas, with each one corresponding to a treatment subgroup. The specimens were either etched with phosphoric acid (control subgroup) or irradiated with erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (subgroups: I-80 mJ, II-120 mJ, and III-180 mJ). Samples were analyzed by micro-EDXRF, yielding three spectra for each area (before and after treatment). Surface mappings covering an area of 80×60 points with steps of 20 μm were also performed on selected specimens. The amount of Ca and P in group A specimens decreased significantly (P<0.05) after the acid etching and the Ca/P ratio increased (P<0.001). Er:YAG laser-etching using lower laser energies did not produce significant changes in dentin components. The mapping data support the hypothesis that acid etching on dentin produced a more chemically homogeneous surface and thus a more favorable surface for the diffusion of adhesive monomers.

Paper Details

Date Published: 1 March 2009
PDF: 7 pages
J. Biomed. Opt. 14(2) 024002 doi: 10.1117/1.3103287
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Luís Eduardo Silva Soares, Univ. do Vale do Paraíba (Brazil)
Ana Maria Do Espirito Santo, Univ. do Vale do Paraíba (Brazil)
Aldo Brugnera, Univ. do Vale do Paraíba (Brazil)
Fátima Antonia Aparecida Zanin, Univ. do Vale do Paraíba (Brazil)
Airton Abrahao Martin, Univ. do Vale do Paraíba (Brazil)

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