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Journal of Biomedical Optics

Effects of Er:YAG laser irradiation and manipulation treatments on dentin components, part 1: Fourier transform-Raman study
Author(s): Luís Eduardo Silva Soares; Ana Maria Do Espirito Santo; Aldo Brugnera; Fátima Antonia Aparecida Zanin; Carolina da Silva Carvalho; Rodrigo de Oliveira; Airton Abrahao Martin
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Paper Abstract

The effects of laser etching, decontamination, and storage treatments on dentin components were studied using Fourier transform (FT)-Raman spectroscopy. Thirty bovine incisors were prepared to expose the dentin surface and then divided in two main groups based upon the decontamination process and storage procedure: autoclaved (group A, n=15) or stored in thymol aqueous solution (group B, n=15). The surfaces of the dentin slices were schematically divided into four areas, with each one corresponding to a treatment subgroup. The specimens were either etched with phosphoric acid (control subgroup) or irradiated with erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (subgroups: I-80 mJ, II-120 mJ, and III-180 mJ, and total energy of 12 J). Samples were analyzed by FT-Raman spectroscopy; we collected three spectra for each area (before and after treatment). The integrated areas of five Raman peaks were calculated to yield average spectra. The areas of the peaks associated with phosphate content (P<0.001), type I collagen, and organic C-H bonds (P<0.05) were reduced significantly in group A (control). Analyses of samples irradiated with reduced laser energies did not show significant changes in the dentin components. These results suggest that thymol storage treatment is advised for in vitro study; furthermore, 12 J of Er:YAG laser energy does not affect dentin components.

Paper Details

Date Published: 1 March 2009
PDF: 7 pages
J. Biomed. Opt. 14(2) 024001 doi: 10.1117/1.3103285
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Luís Eduardo Silva Soares, Univ. do Vale do Paraíba (Brazil)
Ana Maria Do Espirito Santo, Univ. do Vale do Paraíba (Brazil)
Aldo Brugnera, Univ. do Vale do Paraíba (Brazil)
Fátima Antonia Aparecida Zanin, Univ. do Vale do Paraíba (Brazil)
Carolina da Silva Carvalho, Univ. do Vale do Paraíba (Brazil)
Rodrigo de Oliveira, Univ. do Vale do Paraíba (Brazil)
Airton Abrahao Martin, Univ. do Vale do Paraíba (Brazil)


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