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Journal of Biomedical Optics • Open Access

Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements
Author(s): Xiaotao Pan; Xianke Shi; Vladimir Korzh; Hanry Yu; Thorsten Wohland

Paper Abstract

The flow direction of microfluidics in biological applications is not limited to two dimensions, but often extends to three dimensions. Currently there are optical methods available for the measurement of 3-D microfluidic flow vectors, but with low spatial resolution. Line scan fluorescence correlation spectroscopy (FCS) was proposed to determine flow directions in 2-D within microchannels and small blood vessels in our previous work. Importantly, its spatial resolution was demonstrated to be as good as 0.5 μm. In this work, we extend line scan FCS to the third dimension for the characterization of 3-D flow velocity vectors. The spatial resolution is close to the diffraction limit using a scan length of 0.5 μm in all three dimensions. The feasibility of line scan FCS for 3-D microfluidic flow is verified by measurements in microchannels and small blood vessels of zebrafish embryos.

Paper Details

Date Published: 1 March 2009
PDF: 6 pages
J. Biomed. Opt. 14(2) 024049 doi: 10.1117/1.3094947
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Xiaotao Pan, National Univ. of Singapore (Singapore)
Xianke Shi, National Univ. of Singapore (Singapore)
Vladimir Korzh
Hanry Yu, National Univ. of Singapore (Singapore)
Thorsten Wohland, National Univ. of Singapore (Singapore)

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