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Journal of Biomedical Optics

In vivo time-resolved spectroscopy of the human bronchial early cancer autofluorescence
Author(s): Pascal Uehlinger; Tanja Gabrecht; Thomas M. Glanzmann; Jean-Pierre Ballini; Alexandre Radu; Snezana Andrejevic; Philippe Monnier; Georges A. Wagnieres
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Paper Abstract

Time-resolved measurements of tissue autofluorescence (AF) excited at 405 nm were carried out with an optical-fiber-based spectrometer in the bronchi of 11 patients. The objectives consisted of assessing the lifetime as a new tumor/normal (T/N) tissue contrast parameter and trying to explain the origin of the contrasts observed when using AF-based cancer detection imaging systems. No significant change in the AF lifetimes was found. AF bronchoscopy performed in parallel with an imaging device revealed both intensity and spectral contrasts. Our results suggest that the spectral contrast might be due to an enhanced blood concentration just below the epithelial layers of the lesion. The intensity contrast probably results from the thickening of the epithelium in the lesions. The absence of T/N lifetime contrast indicates that the quenching is not at the origin of the fluorescence intensity and spectral contrasts. These lifetimes (6.9 ns, 2.0 ns, and 0.2 ns) were consistent for all the examined sites. The fact that these lifetimes are the same for different emission domains ranging between 430 and 680 nm indicates that there is probably only one dominant fluorophore involved. The measured lifetimes suggest that this fluorophore is elastin.

Paper Details

Date Published: 1 March 2009
PDF: 9 pages
J. Biomed. Opt. 14(2) 024011 doi: 10.1117/1.3088100
Published in: Journal of Biomedical Optics Volume 14, Issue 2
Show Author Affiliations
Pascal Uehlinger, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Tanja Gabrecht, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Thomas M. Glanzmann, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Jean-Pierre Ballini, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Alexandre Radu, Ctr. Hospitalier Univ. Vaudois (Switzerland)
Snezana Andrejevic, Ctr. Hospitalier Univ. Vaudois (Switzerland)
Philippe Monnier, Ctr. Hospitalier Univ. Vaudois (Switzerland)
Georges A. Wagnieres, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


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